KształcenieSzkoła Nauk MedycznychZakład Biologii Komórki i ImmunologiiDepartment of Cell Biology and Immunology

Department of Cell Biology and Immunology

We seek to understand fundamental aspects of: the three somewhat related diseases: (i) cancer; (ii) Graves’ disease, an autoimmune disorder that affects the thyroid gland; (iii) and bacterial infection diseases, and biology of red blood cell. In our study we combine molecular, cellular, and bioinformatics approaches, along with in vivo mouse models, to uncover their mechanisms and therapeutic opportunities. Moreover, we also provide hands-on training in key molecular-based research and diagnostic techniques.

Educational activities

We offer practical courses in molecular biology techniques including:

  • protein detection using one- or two-dimensional (2-D) SDS-PAGE electrophoresis;
  • flow cytometry;
  • immunodetection of proteins, including Western blot;
  • nucleic acid purification, manipulation and detection (e.g., cloning and PCR);
  • microbiology;
  • RT-qPCR.

Scientific activities

In our interdisciplinary research we are especially interested in:

  • autoimmune diseases, with a focus on Graves’ disease and orbitopathy (in vivo mouse model);
  • cancer, particularly multidrug resistance mechanisms in thyroid, glioma and breast cancer;
  • anti-cancer nano-therapies, assessing novel treatment approaches;
  • genome stability, exploring its maintenance and implications for disease progression;
  • infectious diseases, studying E. coli pathogenesis (in vivo mouse model);
  • erythrocyte aging and the role of EV’s in this process.

Research techniques:

  • Cell culture and physiological tests (cell motility and invasiveness, apoptosis, proliferation, drug intake, colony formation, mutagenesis assays and spheroids),
  • Western blot,
  • Immunoprecipitation,
  • ELISA,
  • Gene silencing/overprexpresssion,
  • RT-qPCR,
  • Immunocytochemistry and immunohistochemistry,
  • Flow cytometry,
  • Microbiolocal techniques (transformation, transduction, DNA cloning, site-directed mutagenesis, mutagenesis),
  • In vivo mouse model of urinary tract infection (uroptahogenic E. coli),
  • In vivo mouse model of Graves’ disease and orbitopathy (genetic immunisation).
Scientific cooperations
  • Prof. Paul J. Banga, King’s College London (UK);
  • Prof. Roel M Schaaper, National Institute of Environmental Health Sciences (USA);
  • Prof. Eileen Kennedy, University of Georgia (USA);
  • Prof. Iwona Fijałkowska, Institute of Biochemistry and Biophysics PAS (Poland);
  • Prof. Ewa Nazaruk, Faculty of Chemistry, University of Warsaw (Poland);
  • Prof. Przemysław Juszczyński, Institute of Hematology and Transfusion Medicine (Poland);
  • Prof. Łukasz Łączmański, Laboratory of Genomics & Bioinformatics, Hirszfeld Institute of Immunology and Experimental Therapy PAS (Poland).
Contact:

Centre of Postgraduate Medical Education
Marymoncka 99/103
01-813 Warsaw, Poland 

tel.: (+48) 22 56 93 820
e-mail: kzbb@cmkp.edu.pl

Employees:

Scientists:

Assistant Prof:

Associate Prof:

Research Associate/Research Analyst

PhD Students:

Administrative staff:

Załączniki:
Research grants (PDF, 167 KB)
Publications (PDF, 129 KB)
Successes and awards (PDF, 136 KB)